Figure 5.

Intracellular lipds in three mitotane-resistant vs three nonresistant HAC15 clones. (A) Amounts of phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, phosphatidylethanolamine (PE) based plasmalogens, phosphatidylserine, phosphatidylionositol, lysophosphatidylcholine, ceramide, cholesteryl ester and free cholesterol after treatment with increasing concentrations of mitotane in presence of different serum concentrations. (B) Amounts of saturated, monounsaturated, polyunsaturated, total unsaturated and total cholesteryl ester (CE) as well as free cholesterol (FC), determined as in (A). Treatment with 50 µM mitotane in presence of 5% CCS increases free cholesterol in nonresistant cells but not in mitotane-resistant cells. For lipid amounts and statistical analysis, please refer to Supplementary Tables 5 and 6.