Fig. 6.

Effect of CP4715 on proliferation of IPF lung fibroblasts. a The growth curves of IPF lung fibroblasts. The cells in the absence (solid line) or presence (dashed line) of 1 μM CP4715 dissolved in DMSO were plated at a density of 1.0 × 10⁴ cells/well in 96-well plates. Cell numbers were evaluated at the indicated times. Values are mean ± SD of three independent experiments. *P < 0.05. b IPF lung fibroblasts were treated without (closed circle) or with (closed triangle) 1 μM CP4715 dissolved in DMSO for 24 h. qRT-PCR for the indicated important cell cycle progression genes in CP4715-treated lung fibroblasts were performed. The values were adjusted by GAPDH expression, and the folds for the values are shown. Values are mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, NS: not significant