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. 2020 Jan 30;20:35. doi: 10.1186/s12935-020-1107-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The cytotoxicity of baicalin with the CNE-2R cell lines. a The IC50 values of baicalin on the proliferation of CNE-2R cell lines in different drug influence times. b, c The influence of CNE-2R on apoptosis with different concentrations of baicalin. Apoptosis was identified by Annexin V/PI staining. Early apoptotic cells are revealed in the lower right quadrant of the chart, while the upper right quadrant showed late apoptotic cells (Annexin V +/PI +). The data of histogram was presented as the mean ± standard deviation of 3 replicates. d The impact of baicalin on the proliferation of CNE-2R in 24 h. Cell viability was determined by the CCK-8 assay and expressed with O.D. values. Data were presented as mean ± SD. *P < 0.05 and ***P < 0.001 were contrasted with the control group