Figure 4.
Requirement of integrin αvβ3/β5 for the cross-talk between TGF-β and periostin in lung fibroblasts. (A and B) Effects of knockdown of integrin αV (A) and integrin β3 or β5 (B) on expression of SERPINE1, CTGF, IGFBP3, IL11, POSTN, FN1, and COL1A1. MRC-5 cells were stimulated with or without 3 ng/ml of TGF-β for 24 hours in the presence of control siRNA or integrin αV (ITGAV) or integrin β3 (ITGB3) or β5 (ITGB5) siRNA (n = 3). The values were adjusted by GAPDH expression. The same experiments were performed three times. **P < 0.01.