Figure 2.

P-gp and BCRP protein levels are elevated in the bleo models but not in human idiopathic pulmonary fibrosis (IPF). Mouse and human lung sections were stained with Masson’s trichrome staining. Fibrosis was present in both (A) OP-bleo and SC-bleo models, as well as (B) human IPF. Immunohistochemical staining revealed elevated P-gp protein levels in (C) OP-bleo and in SC-bleo but not in (D) human IPF. Similarly, BCRP protein was increased in (E) OP-bleo and in SC-bleo but not in (F) human IPF. Small-cell carcinoma (SCC) sections had substantial P-gp and BCRP expression, as expected. n = 4 mice per control group and 5 mice per OP-bleo and SC-bleo groups. n = 4 for nonfibrotic human control group, 6 for patients with IPF, 2 per SCC group. Scale bars: 100 μm.