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. 2020 Jan 24;10:6. doi: 10.3389/fcimb.2020.00006

Figure 2.

Figure 2

ICAM-1 expression of keratinocytes co-cultured with MalaEx. (A–F) Confocal microscopy with overlapping fluorescence and phase contrast images showing ICAM-1 expression using Alexa Fluor 488 mouse anti-CD54/ICAM-1 (green) of keratinocytes from donor 1 cultured for 24 h at 37°C with MalaEx at (A) 1 μg/ml, (B) 10 μg/ml, and (C) with M. sympodialis (0.6 × 105 yeast cells/well), (D) with LPS (10 μg/ml) as a positive control, or (E) cultured alone. (F) Keratinocytes cultured alone where the isotype control Alexa Fluor 488 IgG1 has replaced the anti-ICAM-1 antibody. Size bars are 40 μm. (G) The % of ICAM-1 positive keratinocytes defined as strongly positive, after cultured alone or co-cultured with MalaEx (1 or 10 μg/ml) or M. sympodialis (0.6 × 105 yeast cells/well) for 24 h at 37°C. LPS (10 μg/ml) was used as a positive control. Results are presented from three different keratinocyte donors (• Donor 1, ▴ Donor 2, and ■ Donor 3) as mean and standard error of the mean (SEM). Statistically significant differences, as calculated with paired t-test, are indicated in the figure.