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. 2020 Jan 24;10:3048. doi: 10.3389/fmicb.2019.03048

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Copyright © 2020 Azevedo, Mendes and Prudêncio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro activity of ARV compounds on P. berghei sporogonic stages. (A) Timeline of P. berghei sporogonic development and drug incubation periods. (B) Activity of ARV compounds on the conversion of zygotes/gametes into ookinetes. (C) Activity of ARV compounds on oocyst formation. (D) Activity of ARV compounds on oocyst development. All compounds were employed at 10 μM. Bars correspond to RLU measurements represented as the percentage of RLU of the DMSO control. Results are expressed as the mean ± SD. Statistically significant differences between control and treated conditions were analyzed using the Kruskal–Wallis test. N = 3–6. ***P < 0.001; **P < 0.01; *P < 0.05. Detailed statistical analysis is presented in Supplementary Table S2.