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. 2020 Jan 24;11:9. doi: 10.3389/fphys.2020.00009

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Copyright © 2020 Yang, Yang, Liu, Yan, Qiu, Fang, Wang, Werren and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of putative venom proteins in Pachycrepoideus vindemmiae combining transcriptomic and proteomic analyses. (A) 12% SDS-PAGE analysis of P. vindemmiae venom proteins followed by Coomassie Brilliant Blue staining. “M” denotes molecular weight marker. (B) Venn diagram of putative venom proteins combining transcriptomic and proteomic analyses. Pv, P. vindemmiae; Dm, D. melanogaster; VG, venom apparatus (including venom reservoirs and associated glands); Vr, venom reservoir; Vg, venom gland; UVG, upregulated unigenes in VG compared to CA, Proteome: identified unigenes from proteome; SP, unigenes with a secretory signal peptide in their amino acid sequences; NSP, unigenes without a secretory signal peptide in their amino acid sequences. (C) Categories of the 64 putative P. vindemmiae venom proteins based on annotations in the Nr database.