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. 2020 Jan 24;11:9. doi: 10.3389/fphys.2020.00009

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Copyright © 2020 Yang, Yang, Liu, Yan, Qiu, Fang, Wang, Werren and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic reconstruction based on “His_Phos_2 domain” of venom acid phosphatases. The phylogenetic tree was constructed by maximum likelihood method using the program Mega 6. 48 different amino acid substitution models were tested and the “LG+G+I” was considered to be the best model. The bootstrap values are presented on the nodes. Both venom proteins and non-venom orthologs were used for phylogenetic analysis. Full names of abbreviations are listed as follows. Pvin-VAP, P. vindemmiae venom acid phosphatase (Cluster-8535.24978); Pvin-VAPA, P. vindemmiae venom acid phosphatase Acph-1-like (Cluster-8535.6129); Ppup-VAP, P. puparum venom acid phosphatase (ACA60733.1); Nvit-VAPP, N. vitripennis venom acid phosphatase-like precursor (NP_001155147.1); Nvit-VAPA1.1, N. vitripennis venom acid phosphatase Acph-1-like (XP_001600770.1); Lhet-VAPA1.1, L. heterotoma venom acid phosphatase Acph-1-like1 (comp1442_c0_seq1); Lhet-VAPA1.2, L. heterotoma venom acid phosphatase Acph-1-like2 (comp2636_c0_seq1); Lbou-VAPA1, L. boulardi venom acid phosphatase Acph-1-like (comp9544_c0_seq1); Tpre-VAPA1.2, Trichogramma pretiosum venom acid phosphatase Acph-1-like isoform X1 (XP_014234174.2); Fari-VAPA, Fopius arisanus venom acid phosphatase Acph-1-like (XP_011310108.1); Tsar-VAP, T. sarcophagae venom acid phosphatase (OXU23470.1); Mpha-VAPA, Monomorium pharaonis venom acid phosphatase Acph-1-like (XP_012537166.1); Ccin-VAPA, Cephus cinctus venom acid phosphatase Acph-1 (XP_015589422.1); Sinv-VAP, Solenopsis invicta venom acid phosphatase (XP_025987662.1); Veme-VAP1, Vollenhovia emeryi venom acid phosphatase Acph-1-like isoform X1 (XP_011864393.1); Veme-VAP2, V. emeryi venom acid phosphatase Acph-1-like (XP_011872642.1); Aros-VAP, A. rosae venom acid phosphatase Acph-1-like (XP_012251812.1).