TABLE 1.
Venom proteins identified in P. vindemmiae by combined transcriptomic and proteomic analyses.
| Gene ID | VG1 |
VG2 |
VG3 |
Carcass |
Log2 (VG readcount/CA readcount) | NR Description (Blastp) |
||
| FPKM | FPKM | FPKM | FPKM | Accession number | E-value | Putative function | ||
| Hydrolases | ||||||||
| Cluster-8535.6348 | 19557 | 19579 | 97.08 | 11 | 11.081 | XP_008214647.1 | 1.10E−43 | PREDICTED: serine proteinase stubble isoform X2 [Nasonia vitripennis] |
| Cluster-8535.6313 | 836.53 | 957.24 | 220.24 | 0.64 | 11.06 | XP_001599566.1 | 2.50E−54 | PREDICTED: ribonuclease Oy-like [Nasonia vitripennis] |
| Cluster-8535.6305 | 153.65 | 162.55 | 10.17 | 2.18 | 6.7107 | XP_001600074.1 | 1.80E−186 | PREDICTED: serine protease easter [Nasonia vitripennis] |
| Cluster-8535.6218 | 363.86 | 363.47 | 48 | 0 | Inf | XP_008214285.1 | 1.70E−66 | PREDICTED: chymotrypsin-1-like [Nasonia vitripennis] |
| Cluster-8535.6181 | 353.98 | 395.1 | 186.31 | 0 | Inf | NP_001166082.1 | 2.70E−32 | Serine protease 64 precursor [Nasonia vitripennis] |
| Cluster-8535.6139 | 48.33 | 62.27 | 13.84 | 0 | Inf | XP_001600770.1 | 1.00E−104 | PREDICTED: venom acid phosphatase Acph-1-like [Nasonia vitripennis] |
| Cluster-8535.6129 | 709.9 | 861.32 | 129.65 | 0.07 | 14.111 | XP_001600770.1 | 8.40E−126 | PREDICTED: venom acid phosphatase Acph-1-like [Nasonia vitripennis] |
| Cluster-8535.6096 | 787.64 | 840.59 | 55.25 | 0 | Inf | XP_001599566.1 | 1.20E−50 | PREDICTED: ribonuclease Oy-like [Nasonia vitripennis] |
| Cluster-8535.6041 | 722.84 | 819.52 | 290.18 | 0.13 | 13.254 | NP_001155016.1 | 4.90E−86 | Serine protease homolog 29 precursor [Nasonia vitripennis] |
| Cluster-8535.6027 | 392.38 | 479.24 | 22.41 | 0.04 | 13.94 | NP_001166082.1 | 1.30E−47 | Serine protease 64 precursor [Nasonia vitripennis] |
| Cluster-8535.5971 | 476.58 | 559.55 | 322.5 | 0 | Inf | XP_001606746.2 | 2.60E−51 | PREDICTED: venom metalloproteinase 2-like [Nasonia vitripennis] |
| Cluster-8535.5960 | 252 | 261.04 | 32.79 | 0.17 | 11.122 | XP_008216710.1 | 3.70E−170 | PREDICTED: lipase 3-like [Nasonia vitripennis] |
| Cluster-8535.5821 | 203.77 | 241.77 | 103.02 | 0 | Inf | NP_001166092.1 | 8.60E−07 | Serine protease 87 precursor [Nasonia vitripennis] |
| Cluster-8535.25150 | 356.01 | 388 | 123.85 | 0 | Inf | XP_003424313.1 | 8.10E−113 | PREDICTED: LOW QUALITY PROTEIN: xaa-Pro aminopeptidase 1 [Nasonia vitripennis] |
| Cluster-8535.25095 | 135.52 | 168.77 | 38.94 | 0 | Inf | XP_003424313.1 | 1.80E−111 | PREDICTED: LOW QUALITY PROTEIN: xaa-Pro aminopeptidase 1 [Nasonia vitripennis] |
| Cluster-8535.25089 | 270.84 | 86.54 | 74.05 | 0 | Inf | NP_001164348.1 | 4.10E−70 | Serine protease precursor [Nasonia vitripennis] |
| Cluster-8535.25073 | 164.9 | 179.82 | 73.26 | 0.04 | 12.665 | XP_008214271.1 | 2.10E−106 | PREDICTED: chymotrypsin-2-like [Nasonia vitripennis] |
| Cluster-8535.25068 | 300.9 | 349.25 | 22.27 | 0.04 | 13.668 | XP_003424313.1 | 1.70E−111 | PREDICTED: LOW QUALITY PROTEIN: xaa-Pro aminopeptidase 1 [Nasonia vitripennis] |
| Cluster-8535.25024 | 301.44 | 311.46 | 98.65 | 0.5 | 9.9185 | XP_012262898.1 | 4.70E−120 | PREDICTED: neprilysin-like [Athalia rosae] |
| Cluster-8535.25017 | 4271.4 | 4371.7 | 1724.6 | 0 | Inf | NP_001155017.1 | 2.10E−78 | Serine protease 33 precursor [Nasonia vitripennis] |
| Cluster-8535.24980 | 5233.6 | 5127.5 | 704.54 | 0 | Inf | NP_001155087.1 | 3.50E−102 | Endonuclease-like venom protein precursor [Nasonia vitripennis] |
| Cluster-8535.24978 | 536.25 | 533.46 | 19.91 | 0.58 | 10.384 | ACA60733.1 | 3.10E−169 | Venom acid phosphatase [Pteromalus puparum] |
| Oxidordeuctase | ||||||||
| Cluster-8535.6260 | 556.41 | 462.2 | 177.86 | 3.49 | 7.8363 | XP_001600327.1 | 7.60E−225 | PREDICTED: glucose-6-phosphate 1-dehydrogenase [Nasonia vitripennis] |
| Transferase | ||||||||
| Cluster-8535.10302 | 1306.7 | 1291.7 | 47.55 | 0.01 | 16.952 | XP_001607488.2 | 1.70E−261 | PREDICTED: gamma-glutamyltranspeptidase 1 [Nasonia vitripennis] |
| Protease inhibitor | ||||||||
| Cluster-8535.6176 | 97.85 | 125.3 | 19.56 | 0.05 | 11.552 | NP_001164350.1 | 2.30E−23 | Kazal type serine protease inhibitor-like venom protein 2 precursor [Nasonia vitripennis] |
| Recognition/binding proteins | ||||||||
| Cluster-8535.6319 | 1048.3 | 1079.4 | 68.23 | 1.3 | 10.211 | XP_001604854.1 | 5.50E−235 | PREDICTED: low-density lipoprotein receptor-related protein 2-like [Nasonia vitripennis] |
| Cluster-8535.6269 | 118.07 | 99.09 | 62.88 | 0.14 | 10.33 | XP_003425456.1 | 5.30E−108 | PREDICTED: low-density lipoprotein receptor-related protein 2-like [Nasonia vitripennis] |
| Cluster-8535.6043 | 691.26 | 901.49 | 234.82 | 1.7 | 9.5069 | NP_001164343.1 | 2.80E−25 | Chitin binding protein-like venom protein precursor [Nasonia vitripennis] |
| Cluster-8535.25625 | 20.94 | 19.82 | 55.3 | 0 | Inf | XP_003425456.1 | 5.60E−108 | PREDICTED: low-density lipoprotein receptor-related protein 2-like [Nasonia vitripennis] |
| Cluster-8535.25152 | 37.77 | 34.53 | 52.24 | 0 | Inf | XP_003425456.1 | 5.40E−108 | PREDICTED: low-density lipoprotein receptor-related protein 2-like [Nasonia vitripennis] |
| Cluster-8535.25072 | 208.28 | 169.21 | 41.39 | 0 | Inf | XP_003425456.1 | 3.50E−221 | PREDICTED: low-density lipoprotein receptor-related protein 2-like [Nasonia vitripennis] |
| Cluster-8535.24989 | 856.24 | 894.02 | 42.53 | 1.12 | 9.955 | XP_001604854.1 | 1.20E−35 | PREDICTED: low-density lipoprotein receptor-related protein 2-like [Nasonia vitripennis] |
| Others | ||||||||
| Cluster-8535.6175 | 494.74 | 415.13 | 870.57 | 0.05 | 14.205 | NP_001155022.1 | 1.80E−18 | Cysteine-rich/TIL venom protein 2 precursor [Nasonia vitripennis] |
| Cluster-8535.6002 | 206.01 | 173.37 | 110.46 | 0.75 | 8.7008 | XP_001604583.1 | 4.40E−37 | PREDICTED: probable salivary secreted peptide [Nasonia vitripennis] |
| Cluster-8535.5294 | 188.51 | 143.89 | 210.12 | 0.14 | 11.104 | XP_003708569.1 | 9.20E−08 | PREDICTED: venom allergen 3-like [Megachile rotundata] |
| Cluster-8535.13847 | 138.85 | 179.77 | 66.68 | 0 | Inf | XP_003423804.1 | 1.20E−42 | PREDICTED: probable salivary secreted peptide [Nasonia vitripennis] |
| Cluster-8535.12135 | 67.17 | 30.36 | 14.72 | 0.36 | 7.6995 | NP_001154978.1 | 2.80E−149 | Major royal jelly protein-like 9 precursor [Nasonia vitripennis] |
| Unknown proteins | ||||||||
| Cluster-8535.6347 | 286.78 | 376.42 | 39.01 | 0 | Inf | XP_011502473.1 | 3.50E−07 | PREDICTED: uncharacterized protein LOC105365896 [Ceratosolen solmsi marchali] |
| Cluster-8535.6343 | 763.62 | 906.56 | 151.8 | 0.01 | 17.273 | XP_008206401.1 | 5.70E−87 | PREDICTED: uncharacterized protein LOC103316135 [Nasonia vitripennis] |
| Cluster-8535.6342 | 8523.7 | 8690.2 | 3580.7 | 0 | Inf | XP_003426464.2 | 1.50E−23 | PREDICTED: uncharacterized protein LOC100679170 [Nasonia vitripennis] |
| Cluster-8535.6295 | 7027 | 6416.4 | 2015.4 | 0 | Inf | XP_008206613.1 | 1.80E−25 | PREDICTED: venom protein H isoform X1 [Nasonia vitripennis] |
| Cluster-8535.6272 | 636.42 | 745.66 | 221.15 | 0 | Inf | XP_003398548.1 | 3.60E−10 | PREDICTED: uncharacterized protein LOC100649303 [Bombus terrestris] |
| Cluster-8535.6259 | 2842.6 | 2169.4 | 500.37 | 0.05 | 16.055 | XP_003427828.1 | 4.90E−13 | PREDICTED: uncharacterized protein LOC100678638 [Nasonia vitripennis] |
| Cluster-8535.6248 | 1980.5 | 2043.6 | 2440.1 | 0 | Inf | XP_003424286.1 | 1.10E−17 | PREDICTED: uncharacterized protein LOC100678044 [Nasonia vitripennis] |
| Cluster-8535.6242 | 301.5 | 302.67 | 157.3 | 0.02 | 15.009 | XP_008206401.1 | 7.90E−83 | PREDICTED: uncharacterized protein LOC103316135 [Nasonia vitripennis] |
| Cluster-8535.6206 | 915.67 | 1327.3 | 451.62 | 0.16 | 13.398 | XP_001606832.1 | 1.30E−42 | PREDICTED: uncharacterized protein LOC100123223 [Nasonia vitripennis] |
| Cluster-8535.6090 | 1490.1 | 1773.6 | 1235.4 | 0.38 | 12.858 | NP_001155029.1 | 9.00E−19 | Venom protein L precursor [Nasonia vitripennis] |
| Cluster-8535.6065 | 408.8 | 511.71 | 390.53 | 0 | Inf | NP_001155028.1 | 1.30E−13 | Venom protein K precursor [Nasonia vitripennis] |
| Cluster-8535.5978 | 194.01 | 237.28 | 19.94 | 0.07 | 12.229 | XP_001601835.2 | 8.40E−206 | PREDICTED: uncharacterized protein LOC100117668 [Nasonia vitripennis] |
| Cluster-8535.5949 | 251.98 | 157.94 | 283.57 | 0.16 | 11.384 | NP_001164349.1 | 4.50E−69 | Venom protein N precursor [Nasonia vitripennis] |
| Cluster-8535.5822 | 566.96 | 673.72 | 346.48 | 0 | Inf | XP_008214317.1 | 1.20E−39 | PREDICTED: uncharacterized protein LOC103317616 [Nasonia vitripennis] |
| Cluster-8535.25723 | 145.24 | 149.64 | 77.56 | 0.02 | 13.436 | XP_001603409.1 | 5.60E−240 | PREDICTED: uncharacterized protein LOC100119678 [Nasonia vitripennis] |
| Cluster-8535.25580 | 27.42 | 27.49 | 16.69 | 0.2 | 7.8349 | NP_001155170.1 | 9.50E−44 | Venom protein U precursor [Nasonia vitripennis] |
| Cluster-8535.25386 | 81.38 | 101.64 | 22.74 | 0.34 | 8.7077 | XP_001601022.2 | 2.00E−250 | PREDICTED: uncharacterized protein LOC100116563 [Nasonia vitripennis] |
| Cluster-8535.25243 | 38.36 | 37.63 | 10.68 | 0.06 | 9.947 | XP_008217420.1 | 2.60E−14 | PREDICTED: venom protein J isoform X1 [Nasonia vitripennis] |
| Cluster-8535.25157 | 41.59 | 38.61 | 10.18 | 0.36 | 7.4324 | NP_001155170.1 | 1.90E−44 | Venom protein U precursor [Nasonia vitripennis] |
| Cluster-8535.25146 | 216.01 | 280.26 | 82.17 | 0 | Inf | XP_008210187.1 | 1.00E−50 | PREDICTED: uncharacterized protein LOC103316723 [Nasonia vitripennis] |
| Cluster-8535.25086 | 695.81 | 768.91 | 450.62 | 0.03 | 15.253 | NP_001155041.1 | 4.00E−14 | Venom protein V precursor [Nasonia vitripennis] |
| Cluster-8535.25078 | 393.08 | 470.5 | 33.21 | 0.18 | 11.769 | XP_008210187.1 | 3.80E−240 | PREDICTED: uncharacterized protein LOC103316723 [Nasonia vitripennis] |
| Cluster-8535.24993 | 168.07 | 87.31 | 15.91 | 0.04 | 12.065 | XP_001601177.1 | 1.50E−203 | PREDICTED: uncharacterized protein LOC100116763 [Nasonia vitripennis] |
| Cluster-8535.17059 | 1212.9 | 1223.6 | 588.32 | 4.33 | 8.839 | XP_003424551.1 | 1.80E−43 | PREDICTED: uncharacterized protein LOC100680008 [Nasonia vitripennis] |
| Cluster-8535.16907 | 309.8 | 281.19 | 22.68 | 1.87 | 7.8547 | XP_008210468.1 | 2.00E−303 | PREDICTED: uncharacterized protein LOC100680448 isoform X3 [Nasonia vitripennis] |
| Cluster-8535.16090 | 270.75 | 290.36 | 182.01 | 0.38 | 10.333 | XP_001604126.2 | 0.00E+00 | PREDICTED: uncharacterized protein LOC100120484 [Nasonia vitripennis] |
| Cluster-8535.12555 | 124.88 | 120.27 | 64.48 | 0 | Inf | XP_001604126.2 | 0.00E+00 | PREDICTED: uncharacterized protein LOC100120484 [Nasonia vitripennis] |