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Triton X-100 autolysis assays of S. lugdunensis WT and derivative (ΔlytSR and ΔatlL) strains. Bacterial cells were collected from early-exponential growth (OD600 nm = 0.7) and resuspended in an equal volume of buffer containing 0.05% Triton X-100. The rate of autolysis was monitored at OD600 nm. The S. lugdunensis atlL deletion mutant was used as a control for resistance to autolysis. Error bars represent the SD of three independent experiments.
