FIGURE 4.
Bacterial persistence in the apoplast of lettuce. Leaves of intact L. sativa cv. Salinas plants were syringe-infiltrated with bacterial inoculum (1 × 106 CFU/mL). Bacterial titers were followed over a 3-week period and enumerated at 0, 3, 7, 14, and 21 days post-inoculation (dpi) using a serial-dilution plating assay. Results are shown as mean (n = 24 ± SE) obtained from four technical replicates and three biological replicates (i.e., three leaves) repeated twice. The statistical significance between the means (STm 14028s vs. mutant at each time point) was determined using a Student’s t-test (*p < 0.05). Lack of a star on top of the bar indicates no statistical difference.