TABLE 1.

Functional unit annotation for the ten MGD mutant strains selected for further characterization.

Mutant	Plate-well position	Deleted nucleotides	Deletion size (bp)	Deleted gene loci (ID)
Mut1	K_77/78_C08	4538998–4572514	33,516	STM14_RS22490 to STM14_RS22630
Mut2	K_77/78_F03	3618835–3626190	7,355	STM14_RS18330 to STM14_RS18370
Mut3	C_03_H10	2998648–3042149	43,501	STM14_RS15195 to STM14_RS15425
Mut4	C_01_H4	2451061–2455149	4,088	STM14_RS12670 to STM14_RS12690
Mut5	C_01_G2	2016000–2046442	30,442	STM14_RS10460 to STM14_RS10615
Mut6	C_01_F12	1948041–1981245	33,204	STM14_RS10090 to STM14_RS10285
Mut7	C_01_E9	1572754–1583690	10,936	STM14_RS08285 to STM14_RS08335
Mut8	C_01_E6	1534872–1548754	13,882	STM14_RS08105 to STM14_RS08175
Mut9	K_71/72_E4	1462743–1511389	48,646	STM14_RS07705 to STM14_RS07965
Mut10	C_02_G4	1051514–1057804	6,290	STM14_RS05455 to STM14_RS05480

The plate-well position corresponds to the original MGD collection reported by Porwollik et al. (2014). Full annotation of deleted genes is listed in Supplementary Table S3.