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. 2020 Jan 24;10:1385. doi: 10.3389/fgene.2019.01385

Figure 6.

Figure 6

linc00662 regulates the expression of AVL9, but is regulated by miR-497-5p in CRC (A) The predicted 3ʹUTR binding regions of linc00662 in miR-497-5p and the relative luciferase activity in 293T cells following co-transfection with pmirGLO-linc00662-WT or pmirGLO-linc00662-MUT, along with miR-497-5p mimics or NC. (B) miR-497-5p was significantly downregulated in CRC tissues compared to adjacent normal tissues evaluated by RT-qPCR. (C) The relative miR-497-5p expression was upregulated in 69.6% (39/56) CRC patients. (D) Correlation between linc00662 and miR-497-5p at mRNA level in CRC patients. (E) miR-497-5p expression was downregulated in CRC cell lines especially in Caco-2 and SW480 cell lines. (F, G) The expression level of miR-497-5p in linc00662-downregulated CRC cells. (H) The predicted 3ʹUTR binding regions of miR-497-5p in AVL9 and the relative luciferase activity in 293T cells following co-transfection with pmirGLO-AVL9-WT or pmirGLO-AVL9-MUT, along with miR-497-5p mimics or NC. (I, J) The expression level of AVL9 at mRNA level in linc00662/miR-497-5p dysregulated CRC cells. *P < 0.05, **P < 0.001, ***P < 0.001. N.S., No significance.