Figure 4.

CFU assay of SUSD2 ⁺ myometrial and fibroid cells under normoxic and hypoxic conditions. Colony formation assays were performed to assess stem cell activity in normal (20%, Normox) and hypoxic (2%, Hypox) oxygen levels. Representative images are of colonies formed by myometrial (A–D) and fibroid (F–I) SUSD2⁻ (A, B, F, G) and SUSD2⁺ (C, D, H, I) cells grown under Normox (A, C, F, H) or Hypox (B, D, G, I) conditions. Graphs show the rate of colony forming efficiency represented as %CFUs/days for myometrial (E) and fibroid (J) cells. Statistical analysis performed by beta mixed effects with n = 4% transformed matched myometrial and fibroid sample values. Different letters indicate significant differences (P < 0.5). Error bars are +/− SEM.