| Step 1 | 95°C 3 minutes |
| 2 | 95°C 30 seconds |
| 3 | 64°C for CLYBL primers, 58°C for AAVS1 primers, 30 seconds |
| 4 | 72°C 1 minute |
| 5 | repeat (to step 2) 34 times |
| 6 | 72°C 5 minutes |
| 7 | Hold at 12°C |
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