Figure 3:

Binding specificity of Tet1CD and MBD of Mecp2 to methylated and unmethylated DNA. Purified His-Tet1CD or YFP-MBD was incubated with methylated CpG and unmethylated CpG DNA. After 90 min of incubation at 37 °C, free DNA and DNA–protein complexes were separated on a 4.5% native polyacrylamide gel. To quantify the binding, the amounts of unbound DNA (intensities of free DNA bands measured using Image J) were plotted below the gel. Independent experiments were repeated twice. Shown is one representative result. mC:mC, fully methylated CpG containing DNA; C:C, unmethylated CpG containing DNA. Free DNA and protein–DNA complexes are indicated on the gel.