Figure 2.

qRT-PCR for one spike-in control (cel-miR-39) in plasma samples from two donors. (A) Standard curve for spike-in control based on four dilutions spanning a 1000-fold difference in input copy number. (B) Concentration of spike-in control, in million copies per microliters. Note log scale. Both QIAGEN Mini and QIAGEN Serum/Plasma are miRNeasy kits. The QIAGEN Exo kit was not used in this assay. Black and white circles mark samples from two subjects, respectively. UC fractions processed with Zymo Direct-Zol yielded no quantitative results. (C) Overall recovery of spike-in control as percentage of input (equal to 5.6 × 10⁸ copies/sample), in samples as in (B), accounting for elution volume. Black and white circles mark samples from two subjects, respectively.