Table 1.

Genome localization of CpG-enriched region, primer sequences, amplicon size, and potential number of methylation site of each promoter gene

Gene	Position (Peach Genome v2.0)	Primer sequence for bisulphite-treated DNA (5′-3′)	Length (bp)	No. of methylated sites (#mC)
				CpG	CpHpG	CpHpH	Total
DDM1	sd_7:16329264.16329474	F- GCAGAGAGGTATATAAAGTAATTT	211	8	4	9	21
R- CAACCCACCAATA TCTCCATTCAC
CMT3	sd_6:847278.847527	F- GTTTGGTGGAGAGAGAAAGAGGG	250	14	1	7	22
R- TAAAAAAGAAAAT AAAGTTAAAGT
ROS1	sd_7:1472992.14673292	F-TAGAAGAAATTGAAGAGAATAGA	301	9	11	19	39
R- GTAAATATGGTAC AAATAGCAACAC