Figure 3:

Plasmid constructs and biosynthesis of PHB using recombinant E. coli (a) ppct_phaC_Wt Plasmid for Co-expression of PHB producing pct_ap and phaC_cv genes with arabinose inducible araBAD promoter, (b) Over expression of proteins PhaC_cv (mol. Wt.63.27 kDa) and Pct_ap (mol Wt. 55.45 kDa) in the PHB producing recombinant E.coli DH10β transformed with ppct_phaC_Wt recorded on an Agilent 2100 bioanalyzer and 10% SDS-PAGE (c) Fluorescent microscopy of Nile red stained intracellular carbonosomes in recombinant E.coli grown on glycerol supplemented media in the Rhodamine 20 channel (Right) and Rhodamine 20 channel superimposed with TL DIC (Left).