Figure 5:

PHB quantitation from recombinant E. coli. (a) Comparison of PHB quantitation from recombinant E.coli grown on 2 mg/ml glycerol for 24 h using the classical UV spectrophotometric method and the method developed in our study. (b) Over estimation of PHB grown on LB media and LB Media supplemented with glucose using the UV spectrophotometric method. (c) Contributing fluorescence intensities of PHB producing cells grown on LB media without any supplement and LB media supplemented with glucose over estimating PHB. (d) Percentage of over expressed protein to the total protein after 24 h of growth. (e) Ratio of over expressed protein to the total PHB produced after 24 h of growth. (f) PHB quantitation in cell samples after protein digestion.