Table 1:

Performance characteristic of Nile red-based PHB estimation methods using fluorescence spectroscopy

	Accuracy		Precision
Method Detail	%REa	ANOVAb	% RSDc	SEMd (µg/µl)	Sensitivity (Slope)	LODe (µg/ml)	LOQf (µg/ml)	Dynamic
								Range
		F(df)
								(µg/ml)
Sonication	9.59	1657.4, (1, 13)	2.27	7.95	360.12	41.12	124.6	124.6–1000
ethanol extraction
spectrofluorometry		P = 3E-15
Ethanol extraction	14.53	541.6, (1, 13)	4.99	8.58	28.54	46.80	141.83	141.83–600
Spectrofluorometry
		P = 5.5E-12
Sonication spectroflurometry	19.85	229.3, (1, 6)	2.10	2.00	11.70	62.73	190.09	190.09–600
		P = 5.2E-06
UV Spectrophotometery	24.96	1463.6, (1, 11)	2.11	1.01	0.0169	6.15	18.64	18.64–100
		P = 4.7E-13

^aPercentage relative error;

^bAnalysis of Variance;

^cRelative standard deviation;

^dStandard error of mean;

^eLimit of detection;

^fLimit of quantitation.