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. 2018 Aug 8;3(1):bpy007. doi: 10.1093/biomethods/bpy007

Table 2:

Performance characteristics of Nile red-based PHB quantitation in PHB producing E.coli cells compared to UV spectrophotometery

Accuracy
Precision
Method Detail %REa ANOVAb % RSDc SEMd
F(df) µg/µl
Ethanol extraction spectrofluorometry 39.43 21.02, (1, 4) 12.36 4.52
P = 0.019
Protein digestion, 17.22 0.12, (1, 4) 5.531 3.47
 Ethanol extraction
 Spectrofluorometry
P = 0.73
a

Percentage relative error;

b

Analysis of Variance;

c

Relative standard deviation;

d

Standard error of mean.