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. 2020 Jan 13;130(2):877–889. doi: 10.1172/JCI130363

Figure 1. GR forms a complex with FKBP51 via the S211-L225 region of the amino-terminus of GR.

Figure 1

(A) In mouse brain lysate, GR antibody, but not IgG (negative control), coimmunoprecipitated with FKBP51. (B) In mouse brain lysate, FKBP51 antibody, but not IgG (negative control), coimmunoprecipitated with GR. (C) Western blot showing that GST-GRNT, but not GST-GRCT, can “pull-down” FKBP51 in mouse brain tissue. (D) Western blot showing that GST-GRNT4, but not GST-GRNT1, GST-GRNT2, GST-GRNT3, GST-GRNT5, or GST-GRNT6 can pull-down FKBP51 in mouse brain tissue. (E) Western blot showing that GST-GRNT4-1, but not GST-GRNT4-2, GST-GRNT4-3, GST-GRNT4-4, or GST-GRNT4-5, can pull-down FKBP51 in mouse brain tissue. (F) Western blot showing that GST-TPR, but not GST-FK1 or GST-FK2, can pull-down GR in mouse brain tissue. (G) Western blot showing that GST-TPR3, but not GST-TPR1, GST-TPR2, or GST-TPR4 can pull-down GR in mouse brain tissue. (H) Coimmunoprecipitation shows that TAT-GRpep, but not TAT, is able to disrupt the GR-FKBP51 complex in mouse brain slices. Blots represent 3 independent experiments performed.