Figure 3. Injection of TAT-GRpep directly into amygdala reduces fear memory behavior.

(A) A schematic illustration of the experimental schedule. (B) The 2 groups did not differ in the acquisition of cued-fear memory (2-way ANOVA with repeated measures, F_1,21 = 0.25, P = NS). (C) Two-way ANOVA confirmed significant cue effect (F_1,46 = 10.387, P < 0.01), and treatment effect (F_1,46 = 7.708, P < 0.01). The fear memory assessment showed that the treatment peptide (TAT-GRpep) reduced cued-fear memory as the animals injected with TAT-GRpep into the amygdala showed significantly lower level of freezing behavior compared with their control counterparts (P < 0.01); n = 10 (TAT) or n = 13 (TAT-GRpep). (D) For motor cortex injections, the 2 groups did not differ in the acquisition of cued-fear memory (2-way ANOVA with repeated measures (P = NS). (E) The 2-way ANOVA confirmed no significant treatment effect of motor cortex injection (F_1,20 = 0.353, P = NS). The 2 treatment groups did not differ in freezing behavior whether the cue was on or off (P = NS in both cases); n = 10 (TAT) or n = 11 (TAT-GRpep).