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(A–D) HUVECs (A–C) and HHT2 patient–derived BOECs (D) were treated for 3 hours with vehicle (DMSO) or Siro in complete medium (conditioned for 2–3 days) and at the indicated concentrations (A and D) or at 300 nM (B and C), in the absence or presence of LDN-193189 (LDN, 1 μM, B), or the indicated siRNA treatments (C). Cell extracts were analyzed by Western blot using antibodies directed against the indicated proteins.
