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. 2020 Jan 21;130(2):942–957. doi: 10.1172/JCI127425

Figure 11. Siro rescues Smad1/5/8 signaling in the tBMP9/10ib retina.

Figure 11

(A) Scatter plot measuring p-Smad1/5/8–positive area in the retina of P6 control (CTRL) and tBMP9/10ib mice treated with DMSO or Siro, expressed as a percentage of the retinal vascular area occupied by immunostaining. Data represent mean ± SEM (n = 4, 3, and 3 mice for the CTRL, DMSO, and Siro groups, respectively). ***P < 0.001, ****P < 0.0001; 1-way ANOVA, Tukey’s multiple-comparisons test. (B) Representative images showing P6 retinas from tBMP9/10ib mice treated as in A stained with fluorescent isolectin B4 (green), anti-Erg (white), and anti–p-Smad1/5/8 (red). Scale bars: 50 μm. (C) Schematic illustration of the proposed synergistic effects of Siro and Nin on endothelial Smad1/5/8, mTOR, and VEGFR2 signaling pathways.