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. 2019 Dec 17;130(2):e127341. doi: 10.1172/JCI127341

Figure 7. Iron deficiency impairs oxidative phosphorylation in PHTs.

Figure 7

PHTs were treated for 24 hours with 100 μM DFO, apo-Tf, or holo-Tf. (A) Western blotting for OXPHOS complexes CI–CV. β-Actin was used as a loading control. (B) Mitochondrial respiration under basal conditions following injection of oligomycin, the uncoupler FCCP, or the electron transport inhibitors antimycin A and rotenone (AA/ROT). (C) Quantitation of basal respiration, ATP-linked respiration, maximal respiratory capacity, and spare respiratory capacity normalized to total cells per well. Statistical differences between groups were determined by 1-way ANOVA for normally distributed values followed by an all-pairwise multiple comparison (Holm-Sidak method) (###P < 0.001) or 1-way ANOVA on ranks for non-normally distributed values followed by an all-pairwise multiple comparison (Tukey’s test) (#P < 0.05). Lowercase letters indicate a statistical difference compared with DFO (“d”), Apo-Tf (“a”), or Holo-Tf (“h”) group. n = 6 technical replicates. (D) ECAR. (E) Basal OCR versus basal ECAR.