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. 2020 Jan 6;130(2):715–732. doi: 10.1172/JCI126381

Figure 3. Upregulation of the vesicular glutamate release pathway upon stimulation.

Figure 3

(A) mRNA analyses of the enzyme glutaminase and the vesicular transport proteins H-ATPase, Vglut1, and Vglut2 were performed with unstimulated and stimulated (for 4 hours and 24 hours) Th17 cells compared with unstimulated and stimulated Th1 cells (n = 7–15). (B) Western blot analysis of unstimulated and stimulated Th1 and Th17 cells for glutaminase and H-ATPase levels. Representative blots are shown as well as quantification in relation to GAPDH levels (n = 3–4). Data indicate the mean ± SEM. *P < 0.05, by 1-way ANOVA with Tukey’s post hoc test (A) or Mann-Whitney U test (B).