Table 3.
Positive CD8+ T-cell responses to individual S. Typhi proteins.
| Statistic | Protein Secretion | CspA | CspB | CspC | Chp1 | Chp2 | OmpH | OmpL | OmpR | OmpX | TviA | TviB | TviE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total N (N responders*) | 13 (7) | 14 (7) | 14 (7) | 13 (6) | 13 (3) | 15 (7) | 15 (9) | 15 (6) | 15 (7) | 15 (4) | 15 (4) | 15 (4) | |
| % responders | 53.8 | 50.0 | 50.0 | 46.1 | 23.1 | 46.7 | 60.0 | 40.0 | 46.7 | 26.7 | 26.7 | 26.7 | |
| Responders % (N) | IL-17A | 0.0 (0) | 7.1 (1) | 14.3 (2) | 15.4 (2) | 7.7 (1) | 6.7 (1) | 13.3 (2) | 6.7 (1) | 6.7 (1) | 6.7 (1) | 13.3 (2) | 6.7 (1) |
| IFN-γ | 38.5 (5) | 28.6 (4) | 21.4 (3) | 30.8 (4) | 23.1 (3) | 26.7 (4) | 33.3 (5) | 20.0 (3) | 33.3 (5) | 26.7 (4) | 20.0 (3) | 20.0 (3) | |
| TNF-α | 46.2 (6) | 35.7 (5) | 21.4 (3) | 23.1 (3) | 7.7 (1) | 26.7 (4) | 40.0 (6) | 20.0 (3) | 40.0 (6) | 20.0 (3) | 13.3 (2) | 20.0 (3) | |
| CD107a/b | 7.7 (1) | 42.9 (6) | 7.1 (1) | 7.7 (1) | 0.0 (0) | 33.3 (5) | 53.3 (8) | 13.3 (2) | 20.0 (3) | 6.7 (1) | 6.7 (1) | 6.7 (1) | |
*
, Functional responses were considered specific for S. Typhi if the differential in the number of positive and negative events between experimental (Salmonella-Hly proteins) and negative control (Hly only) cultures were significantly increased (P < 0.01) using Z-test. Volunteers were considered responders if the net responses from the PBMC collected 42 days after immunization were greater than 0.1 % from the net responses of PBMC collected before immunization.
Bold fonts represent frequency of responders to a specific protein above 25%.