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. 2020 Jan 21;16(1):e1008591. doi: 10.1371/journal.pgen.1008591

Fig 1. Cells lacking CdnL have pleiotropic morphological and growth defects.

Fig 1

A. Growth of WT (EG865) and ΔcdnL (EG1447) cells in the indicated media. Experiments were performed in triplicate and mean is shown. B. Phase contrast images of WT and ΔcdnL cells grown in the indicated media. Arrows indicate lysed ghost cells. Bar = 2 μm. C. PCA of cell shape for WT and ΔcdnL shown in B. Scatter plots of normalized values (standard deviation (s.d.) from the mean value) for shape modes 1, 2 and 3 which approximately correspond to length, curvature and width. Contours on the left of scatter plots indicate shape of mean and 1 or 2 s.d. from the mean. D. Mean shapes of WT and ΔcdnL cells grown in the indicated media. E. Membrane staining of WT (EG865) and ΔcdnL (EG1447) cells with FM4-64 after growth in HIGG media with and without phosphate. F. Stalk lengths measured using ImageJ’s [43] line tool. Mean and SEM included for plots in C and F. Statistical analysis in C and F performed using one-way ANOVA with Bonferroni’s Multiple Comparison Test, n = 500 for each in C. n = 150, 127, 168, 155, 162, 172 from left to right in E. *** = P < 0.001, * = P < 0.05, ns = not significant.