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. 2020 Jan 21;16(1):e1008433. doi: 10.1371/journal.pgen.1008433

Fig 4. The effects of sorbitol on lignin and callose accumulation upon isoxaben exposure.

Fig 4

(A) Phloroglucinol signal strength indicating lignin accumulation in roots of six-day-old seedlings exposed to mock/300 mM sorbitol or to 600 nM isoxaben/300 mM sorbitol for 12 hours. Genotypes: Col, sub-9 (Col), pUBQ::gSUB:mCherry (line OE SUB-L1), qky-11 (Col). Note absence of detectable signal upon simultaneous application of isoxaben and sorbitol. The experiment was performed three times with similar results (n ≥ 10). (B) Confocal micrographs show cotyledons of seven-day-old sub-9, Col, and pUBQ::gSUB:mCherry (Col, line OE SUB-L1) seedlings treated with mock/300 mM sorbitol or 600 nM isoxaben/300 mM sorbitol for 24 hours. Aniline blue fluorescence signal strength indicates callose accumulation. No increase in signal intensity can be observed in isoxaben-treated seedlings. (C) Quantification of the results depicted in (B). Left panel depicts results obtained from Ler and sub-1 (Ler background). Right panel shows results obtained from Col-0 and sub mutants in Col-0 background and also depicts results from two independent pUBQ::gSUB:mCherry transgenic lines overexpressing SUB (Col, lines SUB OE-L1, SUB OE-O3). Box and whisker plots are shown. Whisker ends mark the minimum and maximum of all the data. 5 ≤ n ≤ 10. The experiment was performed three times with similar results. Abbreviation: ISX, isoxaben. Scale bars: (A) 0.1 mm; (C) 0.2 mm.