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. Author manuscript; available in PMC: 2020 Jan 31.
Published in final edited form as: Immunohorizons. 2019 Jan 15;3(1):13–25. doi: 10.4049/immunohorizons.1800083

FIGURE 5. Modulation of IR by multiple cytokines.

FIGURE 5.

(A) Sorted naive CD4+ and CD8+ T cells were cultured with the indicated cytokines and assessed for expression of PD-L1. (B and C) Sorted naive CD4+ and CD8+ T cells were cultured with anti-CD3/28 stimulation and the indicated cytokines and were assessed for expression of LAG-3 (B) and TIM-3 (C). Representative flow plots for control, IL-27, and IL-12 conditions (left). Summary bar charts (right). (D) TGF-β inhibits IL-27–mediated IR expression in naive CD8+ T cells. Bar charts summarize results of two to four independent experiments. Error bars indicate SEM. Statistical significance of cytokine versus control determined by paired Student t test. ns; p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.