FIGURE 7. IL-27 contributes to IR expression by lung T cells during toxoplasmosis.

(A) Il27p28-GFP reporter mice were infected with 20 Me49 cysts i.p. for 10 d. p28-GFP was examined on nonlymphocytes (CD3⁻NK1.1⁻B220⁻ CD19⁻) within the spleen (black bars) and lung (gray bars). (B) Within nonlymphocytes, p28-GFP expression was examined in macrophages (CD11b⁺CD64⁺Ly6G^loF4/80⁺Ly6C^lo), monocytes (CD11b⁺CD64⁺Ly6G^loLy6C^hi), DC (CD64⁻CD11c⁺MHCII⁺), and polymorphonuclear cells (CD11b⁺CD11c⁻CD64⁻Ly6c^intLy6G⁺) in the spleen (left) and lung (right). (C and D) WT and Il27p28^−/− mice were infected with 20 Me49 cysts i.p. for 11–12 d. IR expression by WT (black bars) and Il27p28^−/− (white bars) lung tetramer⁺ CD4⁺ (B) and CD8⁺ (C) T cells. Three to five mice per group. Data representative of one (A) or four (B and C) independent experiments. Error bars indicate SEM. *p < 0.05, **p < 0.01, ***p < 0.001.