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. 2020 Jan 9;9:e51602. doi: 10.7554/eLife.51602

Figure 6. Superresolution Images of the Distribnution of RyR2 on the Myocyte Surface.

(A) Superresolution images of the surface of isolated cardiomyocytes labeled for the ryanodine receptor under the indicated conditions. Scale bars are 500 nm. (B) Alpha shapes identify individual clusters. Arrows point to examples of subgroups within a cluster. Scalebar is 500 nm. (C) Cumulative distribution functions of the RyR2 cluster sizes. Each treatment group is associated with a different color. (D) Alpha shape drawn around a tetramer array, the tomographic images are from a control myocyte. (E) Cumulative distribution functions of the number of RyR2 within a cluster under the different experimental conditions. Full statistics and the Matlab code are available in Figure 6—source datas 1 and 2 and Source code 2.

Figure 6—source data 1. Statistical Analysis of the Cumulative Distribution Functions for the Alpha Shape Areas.
Figure 6—source data 2. Statistical Analysis of the Cumulative Distribution Functions for the Tetramers per Cluster.

Figure 6.

Figure 6—figure supplement 1. Screen Shot of the Output from the Fourier Ring Correlation Program: Resolution = 18.6 nm.

Figure 6—figure supplement 1.

The image is from the Control experimental group. The original image (white) was split into two (red) which were correlated with each other. For further details, see Banterle et al, J. Struct. Vol 183, pp 363–367, 2013. The contrast of all of the cell images was enhanced for display.