Figure 3. Correlation of the ratios TARPs/PSD-95 and synGAP/PSD-95 among individual animals.

Each point represents mean ratios for a single animal. Corrected ratios and Spearman’s rank correlation coefficients were determined as described under Materials and methods. (A) All animals, including all genotypes, ages, species, and sexes; n = 152. (B) All WT animals, including all ages, species, and sexes; n = 76. (C) All HET animals, including all ages, species, and sexes; n = 76. (D) All female animals, including all genotypes, ages, and species; n = 75. (E) All WT females including all ages and species; n = 36. (F) All HET females, including all ages and species; n = 39. (G) All male animals, including all genotypes, ages, and species; n = 77. (H) All WT males, including all ages and species; n = 40. (I) All HET males, including all ages and species; n = 37. Black symbols, WT; Orange symbols, HET. P-values for Spearman’s rank correlation coefficient are one-tailed. Significant p-values are shown in red.

Figure 3—figure supplement 1. Intensity of PSD-95 bands on immunoblots.

Blots were double-stained for PSD-95 and the target proteins as described under Materials and methods. SynGAP, GluN2B, NLG-1 and NLG-2 were fractionated on 8% gels. TARPS were fractionated on 12% gels. The averaged intensity of staining of PSD-95 was not statistically different between WT and HET (A) or between Male and Female (B), measured on blots of 8% or 12% gels. (C) For each set of blots, a separate gel of the same individual samples was stained for protein with Coomassie blue. For the blots of 7.5 week old mice, synGAP intensity was divided by Coomassie intensity for each individual. PSD-95 intensity (from blots double stained for TARP) was divided by Coomassie intensity for each individual. Plots of synGAP intensity against PSD-95 intensity revealed no significant correlation between the two values. This data shows that the correlation between the synGAP/PSD-95 ration and the TARP/PSD-95 ratio in 7.5 week mice was not influenced by a correlation between levels of synGAP and PSD-95. Separate plots of data from 7.5 week old HET female mice, in which the largest correlation between synGAP and TARP was observed, produced a Spearman’s r = −0.0.2069 with p=0.5187. Plots of data for 7.5 week old male mice also showed no significant correlation.

Figure 3—figure supplement 2. Correlation analysis between synGAP/PSD-95 and TARP/PSD-95 for data from 7 and 12 week old female mice and rats.

Data was acquired as described under Materials and methods and in Figure 3. The small individual data sets indicated in the headings of each panel were normal in distribution. Therefore, Pearson’s r, the parametric equivalent of the non-parametric Spearman’s coefficient, was calculated for each set. (A) A significant inverse correlation was found for 7 and 12 week old female mice, and a strong trend was present for 7 and 12 week old female rats.

Figure 3—figure supplement 3. Correlation analysis between synGAP/PSD-95 and TARP/PSD-95 for data from 7 and 12 week old male mice and rats.

Data was acquired and analyzed as described for Figure 3—figure supplement 2. None of the data sets containing only males showed a significant inverse correlation between levels of TARPs and levels of synGAP. Data from HET mice are shown in blue; data from WT mice are shown in black.