Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
Syngap1 KO mouse | Vazquez et al., 2004 | C57BL/6 | |
| Strain, strain background (Rattus norvegicus) | Syngap1 KO rat; LE-Syngap1em1/PWC | SAGE Labs, Sigma-Aldrich | Syngap1em1/PWC | CRISPR/Cas9 based genome targeting exon 8 |
| Antibody | anti-PSD-95 (Mouse monoclonal) | Caltech Monoclonal Antibody Facility | 50% ammonium sulfate cut of Ascites fluid of 7E3-1B8; AB_212825 | WB (1:10,000) |
| Antibody | anti-synGAP (Rabbit polyclonal) | Pierce | PA1-046; AB_2287112 | WB (1:3,500) |
| Antibody | anti-TARP (Rabbit polyclonal) | EDM Millipore | Ab9876; AB_877307 | WB (1:300) |
| Antibody | anti-GluN2B (Rabbit polyclonal) | Raised in our lab. Zhou et al., 2007, see Figure 2—figure supplement 1 | WB (1:1000) | |
| Antibody | anti-NLG-1 (Rabbit polyclonal) |
Synaptic Systems | 129013; AB_2151646 | WB (1:2000) |
| Antibody | anti-NLG-2 (Rabbit polyclonal) | Synaptic Systems | 129202; AB_2151646 | WB (1:1000) |
| Antibody | Alexa Fluor 680 goat anti-mouse IgG | Thermo Fisher Scientific | A28183; AB_2536167 | WB (1 μg/ml) |
| Antibody | IRdye800-conjugated goat-anti-rabbit IgG | Rockland Immunochemicals, Limerick, PA | 611-145-122; AB_1057618 | WB (1 μg/ml) |
| Commercial assay or kit | BCA Protein Assay Kit | Pierce | 23227 | |
| Software, algorithm | Image Studio Light | LI-COR Biosciences | ||
| Software, algorithm | Microsoft Excel | Microsoft | ||
| Software, algorithm | Prism 8 | GraphPad Software, San Diego |