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. Author manuscript; available in PMC: 2020 Apr 7.
Published in final edited form as: Oncogene. 2019 Oct 7;39(5):1031–1040. doi: 10.1038/s41388-019-1034-9

Figure 1. RBM10 induces apoptosis of colon cancer cells by inducing p53.

Figure 1.

(A) HCT116p53−/− cells were co-transfected with p53 and RBM10 for 48 h. Proteins were analyzed by IB. (B) HCT116p53+/+ and HCT116p53−/− cells were transfected with pcDNA or RBM10 plasmids in different amounts and harvested 48 h post transfection for IB analysis with indicated antibodies. (C) HCT116p53+/+ cells were transfected with RBM10 plasmid and harvested at different time points post transfection for IB analysis with indicated antibodies. (D) HCT116p53+/+ cells were transfected with Flag-RBM10 with or without p53 siRNA, and harvested 48 h post transfection for IB analysis. (E) Cells were transfected with scramble siRNA or RBM10 siRNA and harvested 72 h post transfection for IB analysis with indicated antibodies. (F) HCT116p53+/+ cells were transfected with pcDNA or Flag-p53 and harvested 48 h post transfection for IB analysis with indicated antibodies.