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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Protein Expr Purif. 2019 Nov 29;168:105547. doi: 10.1016/j.pep.2019.105547

Table 2:

Phosphosite mapping of GRK5 purified from E. coli

Peptide (# hits with PTM) GRK5 Range Residue Type Phosphorylation Site Scorea
MELENIVANTVLLK (1) 1–14 Thr 66
DVLDIEQFSTVK (1) 476–487 Thr 74
DVLDIEQFSTVK (1) 476–487 Ser, Thr 51
DVLDIEQFSTVKGVNLDHTDDDFYSK (2) 476–501 Ser, Thr 89
DVLDIEQFSTVKGVNLDHTDDDFYSK (2) 476–501 Ser 77
DVLDIEQFSTVKGVNLDHTDDDFYSK (2) 476–501 Thr 47
TSFNHHINSNHVSSNSTGSSVDHHHHHH (1) 571–590+6His 4x Ser 67
ELFSACAQSVHEYLR (1) 140–154 Cys 45
LGCQEEGAAEVKR (1) 432–444 Cys 79
a

Phosphorylation Site Score indicates both relative abundance and confidence in post-translational modification (PTM) assignment. The largest score for the group of peptides is shown. Phosphorylated residues of the type identified are underlined. Scores ≥40 indicate definitive assignment of the indicated PTM to the corresponding peptide. Multiple hits for the same PTM on a peptide suggest a heterogeneous population.