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Kinetic analysis of GRK5 variants
| Vmax,Rho (fold E. coli GRK5) | Km,Rho (μM) | kcat,Rho/Km,Rho (mM−1*s−1) | Vmax,ATP (fold E. coli GRK5) | Km,ATP (μM) | kcat,ATP/Km,ATP (mM−1*s−1) | |
|---|---|---|---|---|---|---|
| E. coli GRK5 | 1 | 1.8 ± 0.3 | 2.9 ± 1.7 | 1 | 14 ± 4 | 5.9 ± 2 |
| Insect GRK5 | 1.6 ± 0.9 | 0.9 ± 0.2 | 7.3 ± 0.7 | 1.5 ± 0.2 | 26 ± 5 | 7.9 ± 3 |
| E. coli GRK5-S500A | ND | ND | ND | 0.60 ± 0.09 | 20 ± 9 | 4.1 ± 1 |
| E. coli GRK5-T10A | 1.6 ± 0.3 | 1.2 ± 0.4 | 6.5 ± 0.5 | 1.5 ± 0.3 | 14 ± 3 | 8.7 ± 4 |
