Fig. 1.

The expression of c-Rel in the nigrostriatal pathway of MPTP-induced PD mice. (A) Experimental schedule. Luciferin was injected intraperitoneally 15 min ahead of imaging. (B) Representative images were depicted at the time points of −1, 3, 5, 7 and 27 h. The color scale was shown at the right. (C) Relative luciferase activities. n = 5–8 for the time point of 27 h; n = 8–12 for all the other time points. Differences were analyzed by two-way ANOVA followed by LSD multiple comparison tests. ***p < 0.001. (D) c-Rel transcripts in the striatum of mice treated with normal saline and 2 or 4 injections of MPTP. n = 6. (E) Western blot analysis of striatal c-Rel proteins. Quantification of relative c-Rel expression is shown at the bottom. n = 3–4. (F) Western blot analysis of c-Rel proteins in midbrain. Quantification of relative c-Rel expression is shown at the bottom. n = 3–4. NS: group of mice injected with normal saline; M1, M2, M3, M4: groups of mice challenged with one time, two times, three times, or four times i.p. injection of MPTP; 1d: group of mice challenged with four times i.p. injection of MPTP and samples were collected 1 day later. Differences were analyzed by one-way ANOVA followed by LSD multiple comparison tests.*p < 0.05, **p < 0.01, and ***p < 0.001, vs saline-treated control. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)