Fig. 4.

Pro-survival pathways of c-Rel in SH-SY5Y cells. (A) Intracellular ROS indicated by DHE probe in Control, c-Rel shRNA, and c-Rel OE SH-SY5Y cells treated with MPP⁺ or PBS for 24 h. Scale bar: 50 μm. Quantifications of intracellular ROS intensities are shown in the right panel. n = 3–5. Differences were analyzed by one-way ANOVA followed by LSD multiple comparison tests. *p < 0.05, vs PBS groups. (B) Western blot analysis of Bcl-xl, Bcl-2, Bax and SOD2 proteins in control and c-Rel knockdown SH-SY5Y cells. Quantifications of relative Bcl-xl, SOD2 expression and the ratio of Bcl-2 to Bax are shown in the right panel. n = 3. Differences were analyzed by one-way ANOVA followed by LSD multiple comparison tests. **p < 0.01, vs PBS group; #p < 0.05, ##p < 0.01, vs groups of control. (C) Transcripts of Bcl-2, Bcl-xl and SOD2 in control and c-Rel OE SH-SY5Y cells. n = 3. Differences were analyzed by unpaired two-tailed Student's t-test. (D) Western blot analysis of Bcl-2, Bcl-xl and SOD2 proteins in Control and c-Rel OE SH-SY5Y cells. Quantifications of relative Bcl-2, Bcl-xl and SOD2 expression are shown in the right panel. n = 3–4. Differences were analyzed by one-way ANOVA followed by LSD multiple comparison tests. **p < 0.01, vs PBS group; #p < 0.05, ##p < 0.01, vs groups of control. P value in numbers was analyzed by unpaired two-tailed Student's t-test.