Figure 7.

Relationship of miR-34b-5p and miR-892a with Melatonin-Induced Apoptosis and Autophagy

(A) After transfection with miR-34b-5p inhibitor (1 μM) or miR-892a inhibitor (1 μM) for 24 h, melatonin (2 mM) treatment for an extra 24 h. Protein expression were detected by specific primary antibody, respectively. (B) Bar graphs represent the relative density of each band normalized to β-actin. (C) Cells were transfected with miR-34b-5p inhibitor (1 μM) or miR-892a inhibitor (1 μM) for 24 h, and then melatonin (2 mM) treatment for an extra 24 h. Protein expression was detected by specific primary antibody, respectively. (D) Bar graphs represent the relative density of each band normalized to β-actin. (E) After treatment, cells were fixed and stained with DAPI solution. Nuclear fragmentation and condensation were observed under fluorescence microscope. (F) Bar graphs represent the relative density of nuclear fragmentation and condensation. (G) Results were examined under a confocal microscope. All experiments were repeated at least three times. Results are shown as mean ± SEM. *p < 0.05, compared with the control group. #p < 0.05, compared with the only melatonin (2 mM) group.