Figure 3.

miR-7-5p Promotes Osteogenic Differentiation

(A) Quantitative real-time PCR analysis of miR-7-5p expression during osteogenic differentiation. U6 was used as an internal control. (B) Quantitative real-time PCR analysis of Col1a1 and osteocalcin expression. 18S RNA was used as an internal control. (C) Relative miR-7-5p expression in miR-7-5p overexpression cells tested by qRT-PCR. (D) The expression levels of OCN, ALP, Col1a1, and Runx2 detected by qRT-PCR. (E) The expression of Runx2 in miR-7-5p overexpression cells detected by western blot. (F) Alizarin red staining and ALP staining in miR-7-5p overexpression cells compared with control cells. (G) Alizarin red-S staining was quantified by densitometry at 562 nm. (H) Relative ALP activity was measured during osteoblastic differentiation of C3H10 and C2C12 cells. β-actin is a protein-loading control. All assays were repeated at least three times. The data are the mean ± SD (n = 3). n.s., not significant, *p < 0.05, **p < 0.01, ***p < 0.001.