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. 2020 Jan 31;11:640. doi: 10.1038/s41467-020-14281-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The inserted image shows the expression of hChR2-eGFP in the caudal segment of the left aIC. Scale bar, 250 μm. b Raster plot of approaches to food in 24-h fasted mice expressing hChR2-eGFP in the caudal segment of the left aIC and receiving 20 Hz photostimulation. c Total food consumption (left), number of approaches (middle) and the percentage of feeding time (right) were measured (n = 7 mice per group, Two-tailed unpaired t test). d Representative locomotor trace of a mouse with the caudal segment of the left aIC^CamKII neurons expressing eGFP or hChR2 and receiving 20 Hz photostimulation in the laser compartment. e Percentage of time spent in the photostimulation side (left) and locomotor activity (right) in laser or no laser sides (n = 7 mice per group, Two-tailed unpaired t test). Data are presented as means ± SEM. Source data are provided as a Source Data file.