Fig. 5. Inhibition of the right aIC^CamKII neurons rescues Cisplatin-induced anorexia.

a The inserted image shows the expression of hM3Dq-mCherry in the caudal segment of the right aIC. Scale bar, 250 μm. b Diagrams illustrating the injected virus (upper) and example current-clamp traces from the brain slice with the right aIC^CamKII neurons expressing hM3Dq before and after treatment with CNO. c Food intake (left) and percentage of feeding time (right) in CNO-treated 24 h-fasted mice expressing hM3Dq in the right aIC^CamKII neurons during 20, 40, or 60-min test (n = 7 mice for per group). d Chronic administration of CNO (pink area, 1 mg/kg, every 12 h for 7 days, i.p.) reduces body weight (n = 8 mice per group). e The inserted image shows the expression of hM3Di-mCherry in the caudal segment of the right aIC. Scale bar, 250 μm. f Diagrams illustrating the injected virus (upper) and current–voltage relationship of a representative aIC^CamKII neuron recorded before and after perfusion with CNO. Raw traces show individual voltage responses to a series of 500 ms current pulses from 100 to 200 pA with 10-pA steps. Red traces indicate the minimal current to induce action potentials. g Food intake in 24 h-fasted mice after administration of different anorexigenic agents (CCK (5 μg/kg), LiCl (150 mg/kg), and LPS (0.1 mg/kg) and CNO (n = 8 for left-mCherry, left-hM4Di, and right-mCherry group, n = 6 for right-hM4Di group). h Chronic administration of CNO (pink area, i.p.) increases body weight (n = 9 mice for control group, n = 8 mice for hM4Di group). i Chronic administration of Cisplatin (blue area, 4 mg/kg, i.p.) every 2 days for 14 days followed by CNO injection (green area, i.p.) every 12 h for 7 days after 7 days Cisplatin injection. Body weight of mice was measured every day (n = 8 mice per group). Two-way ANOVA with Bonferroni post hoc analysis. *P < 0.05; **P < 0.01; ***P < 0.005. Data are presented as means ± SEM. Source data are provided as a Source Data file.