Fig. 6. Right aIC^CamKII neurons project to the LH directly.

a Representative histology of images from the LH showing CamKII⁺ fiber terminal in green after injecting hSyn-DIO-eGFP into the right aIC of Camk2a-Cre mice. Distribution of the fiber terminals in the area of hypothalamus is shown from bregma −0.70 mm to bregma −1.94 mm. Scale bar, 250 μm. AHA anterior hypothalamic area, anterior part; AHC anterior hypothalamic area, central part; Arc arcuate hypothalamic nucleus; f fornix; LA lateroanterior hypothalamic nucleus; 3V 3rd ventricle; ic internal capsule; SI substantia innominate; AHP anterior hypothalamic area, posterior part; MTu medial tuberal nucleus; VMH ventromedial hypothalamic nucleus; MCLH magnocellular nucleus of the lateral hypothalamic; DM dorsomedial hypothalamic nucleus; PeF perifornical nucleus; PaLM paraventricular hypothalamic nucleus, lateral magnocellular part; SCh suprachiasmatic nucleus; MCPO magnocellular preoptic nucleus. b eGFP or hChR2-eGFP injected in the right aIC and the terminals in LH were activated by light and immunostained with Fos (red) 30 min after light stimulation (n = 5 mice per group, Two-way ANOVA with Bonferroni post hoc analysis). Scale bar, 200 μm. c Light can evoke EPSCs (held at −70 mV), but not IPSCs (held at 0 mV). n = 6 cells. d Single light evoked EPSC, which can be blocked by CNQX. n = 5 cells. e Diagram illustrating the injected virus (AAV-FLEX-eGFP-2A-TVA, AAV-FLEX-RVG, and ΔRVG-DsRed) (upper) and injection sites (lower) for rabies-based projection-specific monosynaptic tracing of inputs to LH^vGluT2 neurons in Slc17a6-ires-Cre mice. Rabies are colocalized with CamKII in the right aIC (upper). Neurons co-infected (starter cell) with helper AAV and rabies are shown in yellow. f Distribution of monosynaptic input (Rabies-DsRed neurons) from the right aIC to the LH^VGluT2. Scale bar, 100 μm (Right upper). Scale bar, 25 μm (Right lower). Source data are provided as a Source Data file.