Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 31;10:1546. doi: 10.1038/s41598-020-58150-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Binding-analysis of monospecific antibody fragments. (a) Screening in FMAT of individual soluble scFv showing the binding to CD40 expressing cells versus binding to mock transfected cells. (b) Binding of unique clones to CD40 expressing cells in the first screening versus their binding after re-expression. (c) Binding of clones in ELISA to coated recombinant CD40 and a non-related protein carrying the same tag as the target (non-target). (d) Screening of VH-VLD Fab clones in ELISA for binding to target versus non-target protein. Yellow-marked clones were selected as binders and used for Sanger sequencing to identify unique sequences.