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. 2020 Jan 31;11:632. doi: 10.1038/s41467-020-14377-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c The SDS-PAGE protein gel analysis of the His₆-sumo-rA3G WT and various mutants after nickel affinity column purification (a), and 20% denaturing urea polyacrylamide gel analysis of RNAs associated with the proteins without RNase A treatment during purification (b) or with RNase A treatment during purification (c) (see methods for details). d, e Superdex-200 size exclusion chromatography (SEC) analysis of the sumo-rA3G WT and mutant proteins before (d) and after (e) RNase A treatment. The positions corresponding to void volume, dimer, and monomer are indicated with arrows. Source data for all panels are provided in the Source Data file.