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. 2020 Jan 31;11:632. doi: 10.1038/s41467-020-14377-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Immunoblotting with FLAG antibody was used to detect transfected A3G wild type and mutants expressed in 293T virus producer cells and encapsidated into VSV-G pseudotyped virions in the absence, ΔVif (A) or presence, +Vif (B) of the A3 antagonist, Vif. The cell lysate and virion loading controls were α-tubulin and p24, respectively. The relative A3G levels shown below blots were calculated by setting the A3G wild type condition to 1 and determining the relative values of other lanes. One representative blot from three independent experiments is shown. c Infectivity in the absence or presence of Vif was measured by β-galactosidase activity in TZM-bl reporter cells. Results were normalized to the no A3 condition. Error bars represent the standard deviation of the mean calculated from three independent experiments. d The relative amount of proviral DNA integration in infected 293T cells in the presence of A3G wild type and mutants in comparison to the No A3 condition was determined by qPCR. Error bars represent the standard deviation of the mean calculated from at least two independent experiments. e The result of deaminase activity assay in lysates from 293T cells expressing hA3G and mutants. Error bars represent the standard deviations of the mean calculated from three independent experiments. Source data are provided in the Source Data file.